National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Vývoj konstruktu umožňujícího sledování veškerých G proteinů v buňce pomocí dvoufotonové polarizační mikroskopie
SVOBODOVÁ, Karolína
The aim of this work was creation of a construct that could act as a universal sensor of G-proteins activation. The work included construct design and preparation, as well as expression in mammalian cells and observations by two-photon polarization microscopy. The thesis includes a summary of current ways of protein interaction detection, an explanation of two-photon microscopy, a description of molecular-biological methods used for construct preparation, and outcomes of our microscopy observations.

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